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Molecular Biology Reagents

 

   

 

 
Agarose, Low Melting (Molecular Grade) 

 

 

 

Description

 

Top VisionTM LM GQ Agarose is highly purified agarose with a low melting temperature certified by strict quality control test procedures.

Specification
· Electroendosmosis EEO <0.12
· Gel strength (1% gel) >250g/cm2
· Gelling temperature (1.5% gel) 24-28°C
· Melting temperature (1.5% gel) <65.5°C
· Moisture <7%
Application

· Preparation of DNA preparative gels in routine molecular biology techniques
· In-gel enzymatic processing (digestion, ligation etc.)
· Digestion of remelted agarose by agarase enzymes makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing
· The DNA recovered from agarose gels after electrophoresis can be used in enzymatic processes
(restriction, ligation, etc.)

Notes

· May be used at concentrations between 0.8-2% with all typical buffer systems.
· Low DNA binding.

 

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