| Protein
Molecular Weight Marker |
| Description |
The Protein Molecular
Weight marker is a mixture of 7 purified proteins supplied
in gel loading buffer for direct application to an SDS-polyacrylamide
gel (SDS-PAGE) the proteins resolve into sharp, tight
bands in the range of 14.4kDa to 116.0kDa when analyzed
by SDS-PAGE and stained with Coomassie Brilliant Blue
R-250.
| Protein |
Source |
Molecular weight, kDa |
| beta-galactosidase |
E.coli |
116.0 |
| Bovine Serum albumin |
bovine plasma |
66.2 |
| Ovalbumin |
chicken egg white |
45.0 |
| Lactate dehydrogenase |
porcine muscle |
35.0 |
| RE Bsp98I |
E.coli |
25.0 |
| beta-lactoglobulin |
bovine milk |
18.4 |
| Lysozyme |
chicken egg white |
14.4 |
|
| Contents |
0.1-0.2mg/ml of each protein
listed below. |
| Storage
Buffer |
· 62.5mM Tris-HCl
(pH 7.0 at 25°C)
· 1mM EDTA
·
2% SDS
· 50mM DTT
· 30mM NaCl
· 1mM NaN3
· 0.01% bromophenol blue
· 50% glycerol |
| Note |
Applicable gel concentration:
8-15% (37.5:1 Acrylamide:Bis-Acrylamide). |
