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BamHI
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BamHI is a restriction enzyme which recognizes the following sequence:
5'...G▼GATCC...3'
3'...CCTAG▲G…5'
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Concentration
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10u/μl
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Reaction condition |
Recommended Buffer: Buffer BamHI+
10mM Tris-HCl (pH 8.0), 5mM MgCl2, 100mM KCl, 0.02% Triton X-100 and 0.1mg/ml BSA. |
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Incubation temperature: 37°C |
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Thermal inactivation: 65°C |
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Source
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Bacillus amyloliqufacience
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Frequency of Cutting
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| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
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| 5 | | 0 | 1 | 1 | 1 |
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Storage
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-20°C
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Storage
buffer
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10mM Tris-HCl (pH 7.4
at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol
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Ligation
and Recleavage
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After 50-fold overdigestion
with BamHI, approximately more than 90% of the DNA fragments
can be ligated and recut
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Methylation
Effect
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BamHI does not cut GGATm4CC,
GGATm5CC,
GGAhm5UCC, but cuts GGATCm5C, GGm6ATCC,
GGm6ATCm5C and GGATCm4C |
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