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BclI
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BclI is a restriction enzyme which recognizes the following sequence:
5'...T▼GATCA...3'
3'...ACTAG▲T…5'
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Concentration
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10u/μl
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Reaction condition |
Recommended Buffer: Buffer G+
10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml
BSA. |
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Incubation temperature: 55°C |
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Thermal inactivation: 80°C |
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Source
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Bacillus caldolyticus.
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Frequency of Cutting
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| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
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| 8 | 5 | 0 | 0 | 0 | 0 |
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Storage
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-20°C
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Storage
buffer
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10mM Tris-HCl (pH 7.4
at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol
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Ligation
and Recleavage
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After 50-fold overdigestion
with BclI, approximately more than 90% of the DNA fragments
can be ligated and recut
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Methylation
Effect
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BclI cuts neither TGm6ATCA
nor TGAThm5CA, but cuts TGATm5CA.
Blocked by Dam methylation |
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