Restriction Enzymes

 

BglII     BglII is a restriction enzyme which recognizes the following sequence: A▼GATC T T CTAG▲A Concentration   5u/µl   Reaction condition Recommended Buffer: Buffer O+ 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA . Incubation temperature: 37°C Inactivation: 10mM EDTA   Source   Bacillus globigii.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 6 11 0 0 1 0   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with BglII, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   BglII does not cut AGATm5CT and AGAThm5CT, but cuts AGm6ATCT and AGAhm5UChm5U       Order   Back