Restriction Enzymes

 

DraI (AhaIII)     DraI is a restriction enzyme which recognizes the following sequence: TTT▼AAA AAA▲TTT Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer B+ 10mM Tris-HCl (pH 7.5), 10mM MgCl2, and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 65°C   Source   Deinococcus radiophilus.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 13 12 2 3 5 3   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with DraI, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   DraI does not cuts TTTAm6A. Blocked by overlapping EcoKI methylation       Order   Back