Restriction Enzymes

 

EcoRV (Eco 321)     EcoRV is a restriction enzyme which recognizes the following sequence: GAT▼ATC CTA▲TAG Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer R+ 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 65°C   Source   Escherichia coli J62 pLG74.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 21 9 0 0 0 1   Storage   -20°C   Storage buffer   25mM Tris-HCl (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with EcoRV, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   EcoRV (Eco 321) cuts GATATm5C       Order   Back