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HaeIII
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HaeIII is a restriction enzyme which recognizes the following sequence:
GG▼CC
CC▲GG
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Concentration
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10u/μl
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Reaction condition |
Recommended Buffer: Buffer R+
10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA . |
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Incubation temperature: 37°C |
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Thermal inactivation: 80°C |
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Source
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Haemophilus aegyptius.
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Frequency of Cutting
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| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
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| 149 | 219 | 11 | 11 | 15 | 22 |
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Storage
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-20°C
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Storage
buffer
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10mM Tris-HCl (pH 7.5
at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml
BSA and 50% glycerol
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Ligation
and Recleavage
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After 50-fold overdigestion
with HaeIII, approximately more than 90% of the DNA fragments
can be ligated and recut
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Methylation
Effect
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HaeIII does not cut GGm5CC,
but cuts GGCm5C
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