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HhaI
(Hin6I)
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HhaI is a restriction enzyme which recognizes the following sequence:
G CG▼C
C▲GC G |
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Concentration
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10u/μl
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Reaction condition |
Recommended Buffer: Buffer Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA. |
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Incubation temperature: 37°C |
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Thermal inactivation: 80°C |
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Source
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Haemophilus haemolyticus.
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Frequency of Cutting
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| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
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| 215 | 375 | 18 | 17 | 26 | 31 |
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Storage
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-20°C
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Storage
buffer
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10mM potassium phosphate
(pH 7.4 at 25°C), 100mM NaCl, 1mM EDTA, 7mM 2-mercaptoethanol,
0.2mg/ml BSA, and 50% glycerol
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Ligation
and Recleavage
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After 50-fold overdigestion
with HhaI, approximately more than 90% of the DNA fragments
can be ligated and recut
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Methylation
Effect
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HhaI (Hin6I) does not cut
Gm5CGC
Blocked by CG methylation
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