Restriction Enzymes

 

MboI     MboI is a restriction enzyme which recognizes the following sequence: ▼GATC CTAG▲ Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer R+ 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 65°C   Source   Moraxella bovis .   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 116 87 0 15 7 22   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with MboI, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   MboI cuts neither Gm6ATC, GAThm5C nor GAhm5UC, but cuts GATm4C and GATm5C Blocked by Dam methylation   Notes Sensitive to dam methylation.     Order   Back