Restriction Enzymes

 

MspI (HpaII)     MspI is a restriction enzyme which recognizes the following sequence: C▼CG G G GC▲C Concentration   10u/μl   Reaction condition Recommended Buffer: Y+/TANGO™ 33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 65°C   Source   Moraxella sp.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 328 171 5 13 18 26   Storage   -20°C   Storage buffer   10mM potassium phosphate (pH 7.5 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol Ligation and Recleavage   After 50-fold overdigestion with MspI, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   MspI (HpaII) does not cut m4CCGG, m5CCGG, Cm4CGG and hm5 Chm5CGG, but cuts Cm5CGG       Order   Back