Restriction Enzymes

 

NdeI     NdeI is a restriction enzyme which recognizes the following sequence: CA▼TA TG GT AT▲AC Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer R+ 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 65°C   Source   Neisseria denitrificans.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 7 2 0 1 3 1   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with NdeI, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   NdeI does not cut CATm6ATG, but cuts m5CATATG       Order   Back