Restriction Enzymes

 

PvuII     PvuII is a restriction enzyme which recognizes the following sequence: CAG▼CTG GTC▲GAC Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer G+ 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 80°C   Source   Proteus vulgaris.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 15 24 0 2 3 1   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with PvuII, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   PvuII cuts neither CAGm4CTG nor CAGm5CTG   Note A large excess of the enzyme results in star activity     Order   Back