Restriction Enzymes

 

SalI     SalI is a restriction enzyme which recognizes the following sequence: G▼TCGA C C AGCT▲G Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer O+ 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 65°C   Source   Streptomyces albus G.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 2 3 0 1 1 1   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 7.5 at 25°C), 100mM KCl, 10mM 2-mercaptoethanol, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with SalI, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   SalI does not cut GTm5CGAC, GTCGm6AC, Ghm5UCGAC, but cuts GTCGAm5C Blocked by CG methylation   Note A large excess of the enzyme results in star activity     Order   Back