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SmaI
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SmaI is a restriction enzyme which recognizes the following sequence:
CCC▼GGG
GGG▲CCC
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Concentration
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10u/μl
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Reaction condition |
Recommended Buffer: Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM K-acetate
and
0.1mg/ml BSA. |
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Incubation temperature: 30°C |
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Thermal inactivation: 65°C |
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Source
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Serratia marcescens.
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Frequency of Cutting
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| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
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| 3 | 12 | 0 | 1 | 1 | 0 |
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Storage
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-20°C
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Storage
buffer
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10mM Tris-HCl (pH 7.4 at
25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA
and 50% glycerol
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Ligation
and Recleavage
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After 50-fold overdigestion
with SmaI, approximately more than 90% of the DNA fragments
can be ligated and recut
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Methylation
Effect
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SmaI does not cut m4CCCGGG, m5CCCGGG,
Cm4CCGGG, CCm4CGGG, CCm5CGGG, but cuts Cm5CCGGG
Blocked by CG methylation
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Notes |
A large excess of the enzyme
results in star activity
Incubation at 37°C
results in 50% activity |
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