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TaqI
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TaqI is a restriction enzyme which recognizes the following sequence:
T▼CG A
A GC▲T |
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Concentration
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10u/μl
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Reaction condition |
Recommended Buffer: Buffer TaqI+
10mM Tris-HCl (pH 8.0), 5mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. |
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Incubation temperature: 65°C, under paraffin oil in a capped vial |
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Thermal inactivation: No |
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Source
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Thermus aquaticus YTI.
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Frequency of Cutting
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| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
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| 121 | 50 | 10 | 4 | 13 | 7 |
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Storage
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-20°C
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Storage
buffer
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10mM Tris-HCl (pH 7.5 at
25°C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA
and 50% glycerol
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Ligation
and Recleavage
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After 50-fold overdigestion
with TaqI, approximately more than 90% of the DNA fragments
can be ligated and recut
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Methylation
Effect
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TaqI does not cut TCGm6A
and Thm5CGA, but cuts Tm5CGA
Blocked by overlapping Dam methylation
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Note |
Assayed using Lambda DNA
(dam-)
Incubation at 37°C results in 10% activity |
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