|
|
XbaI
|
|
|
XbaI is a restriction enzyme which recognizes the following sequence:
T▼CTAG A
A GATC▲T |
 |
|
Concentration
|
10u/μl
|
|
Reaction condition |
Recommended Buffer: Y+/TANGO™
33mM Tris-acetate (pH 7.9), 10mM Mg-acetate, 66mM K-acetate
and
0.1mg/ml BSA. |
|
Incubation temperature: 37°C |
|
Thermal inactivation: 65°C |
|
|
|
Source
|
Xanthomonas badrii.
|
|
Frequency of Cutting
|
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|
| 1 | 5 | 0 | 1 | 1 | 0 |
|
|
Storage
|
-20°C
|
|
Storage
buffer
|
10mM Tris-HCl (pH 7.5 at
25°C), 100mM KCl, 10mM
2-mercaptoethanol, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol
|
|
Ligation
and Recleavage
|
After 50-fold overdigestion
with XbaI, approximately more than 90% of the DNA fragments
can be ligated and recut
|
|
Methylation
Effect
|
XbaI does not cut TCTAGm6A,
Tm5CTAGA nor does it cut Thm5CTAGA
Blocked by overlapping Dam Methylation
|
|
Note |
Assay on Lambda DNA (dam-) |
|
|
|
| |
|
|
