Restriction Enzymes

 

XhoI     XhoI is a restriction enzyme which recognizes the following sequence: C▼TCGA G G AGCT▲C Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer R+ 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA . Incubation temperature: 37°C Thermal inactivation: 80C   Source   Xanthomonas holcicola.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 1 6 1 0 0 0   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with XhoI, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   XhoI does not cut CTCGm6AG and m5CTCGAG CTm5CGAG is cleaved by XhoI at a significantly slower rate CG methylation may influence DNA cleavage       Order   Back