Restriction Enzymes

 

PstI     PstI is a restriction enzyme which recognizes the following sequence: C TGCA▼G G▲ACGT C Concentration   10u/μl   Reaction condition Recommended Buffer: Buffer O+ 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Incubation temperature: 37°C Thermal inactivation: 80°C   Source   Recombinant E. coli strain.   Frequency of Cutting   λ Ad-2 ΦX174 pUC18 M13mp18 pBR322 28 30 1 1 1 1   Storage   -20°C   Storage buffer   10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% glycerol   Ligation and Recleavage   After 50-fold overdigestion with PstI, approximately more than 90% of the DNA fragments can be ligated and recut   Methylation Effect   PstI cuts neither m5CTGCAG, Chm5UGCAG, CTGm5CAG nor CTGCm6AG       Order   Back